Research Technician 2-Surgery (Knoxville)
University of Tennessee, Knoxville
Knoxville, TN
Full-time
Research
Posted on April 15, 2024
Market Range: 05
Hiring Salary: $19.10/Hourly
THIS IS A GRANT-FUNDED POSITION
JOB SUMMARY/ESSENTIAL JOB FUNCTIONS: The Research Technician 2 assists with conducting experiments related to inflammation, acute lung injury, islet transplantation, nutrition, and sepsis research. Additional duties include assisting with the establishment of a research program to foster basic research; organizing and maintaining laboratory equipment; helping to analyze, create, organize, and plot experiments; conducting literature searches, and giving research presentations.
DUTIES AND RESPONSIBILITIES:
- Performs animal related duties including anesthesia administration, assisting in microsurgical procedures, pre-operative and post-operative care, blood collection, distribution of special diet as needed, weighing of animals, general animal handling, animal husbandry, and maintaining daily care notebook and surgical procedure notebook.
- Conducts molecular and cellular biology assays, such as Western blot analysis, ELISA (enzyme-linked immunosorbent assay), and immunohistochemistry in accordance with standard operating procedures.
- Maintains viable tissues and cell cultures for in vitro experiments and performs phenotype response assays on living cultures.
- Feeds cultures to maintain viability, passages cell lines and splits culture populations, prepares culture populations for long-term storage in liquid nitrogen, administers experimental treatments to culture environments, and measures responsive elements, as appropriate.
- Performs data acquisitions and analyses and summarizes the results of all experiments in weekly lab meetings.
- Presents experimental outcomes that aid in the preparation of manuscripts and grant proposal applications.
- Prepares buffers and experimental reagents, washes glassware and non-consumable experimental supplies, maintains clean/organized flammable safety cabinets and chemical storage cabinets, autoclaves glassware, sterilizes and maintains microsurgical instruments, and provides general maintenance of laboratory bench areas and other workspaces.
- Orders laboratory supplies and research animals.
- Performs other related duties as assigned.
Qualifications :
MINIMUM REQUIREMENTS:
EDUCATION: Bachelor’s Degree in Biological Science, Chemistry, or Science-related field. (TRANSCRIPT REQUIRED)
EXPERIENCE:OR a combination of education, training, and experience to equal eight (8) years. Must be able to work with small animals/rodents. Must maintain IACUC (Institutional Animal Care & Use Committee) approval to use animals in research. (Knowledge of animal protocol regulations, microscopy, and statistics and experience in biological research laboratory experience are preferred).
KNOWLEDGE, SKILLS, AND ABILITIES:
- Demonstrates an understanding IACUC rules and regulations.
- Ability to implement rules and regulations within the laboratory.
- Knowledge of standard cellular and molecular biology principles and basic science techniques.
- Ability to implement knowledge of biological assays in an effective manner in practice.
- Ability to assist in laboratory animal activities (survival and non-survival).
- Ability to be effective in data analysis and presentation using Statistical Software (GraphPad), Excel, and PowerPoint platforms.
- Skilled in standard laboratory techniques.
